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KMID : 0355420160400010003
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Journal of Korean Academy of Oral Health
2016 Volume.40 No. 1 p.3 ~ p.8
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Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois
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Shin Ae-Ri
Ohk Seung-Ho
Choi Choong-Ho
Hong Suk-Jin
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Abstract
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Objectives: This study aims to identify and partially purify antibacterial compounds against Streptococ-cus mutans from Galla Rhois extract.
Methods: Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper disc-diffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC).
Results: The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using parti-tion chromatography and HPLC, and the antibacterial activity was confirmed.
Conclusions: The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.
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KEYWORD
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Antibacterial compounds, Column chromatography, Galla Rhois, Partition chromatogra-phy, Streptococcus mutans, Thin-layer chromatography
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